target protein specific antibody  (Proteintech)

Journal: Frontiers in Oncology

Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

doi: 10.3389/fonc.2025.1538649

Figure Lengend Snippet: Knockdown of OSMR inhibits activation of JAK/STAT3/CCL-2 signaling pathway in tumor cells. WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis. N = 3, ****p < 0.0001.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

Techniques: Knockdown, Activation Assay, Expressing

Journal: Frontiers in Oncology

Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

doi: 10.3389/fonc.2025.1538649

Figure Lengend Snippet: OSMR activates JAK/STAT3/CCL-2 pathway to promote malignant behavior of tumor cells. (A) WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis; (B) CCK-8 detection of cell proliferation; (C) Colony formation experiment to detect cell proliferation; (D) Transwell detects cell invasion ability; (E) Scratch test to detect cell migration ability; (F-G) Flow cytometry is used to detect cell apoptosis rate (F) and cell cycle (G) . N = 3, ***p < 0.001, ****p < 0.0001.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

Techniques: Expressing, CCK-8 Assay, Migration, Flow Cytometry

Journal: Frontiers in Oncology

Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

doi: 10.3389/fonc.2025.1538649

Figure Lengend Snippet: OSMR/JAK/STAT3/CCL-2 pathway regulates malignant behavior of tumor cells and induces M2 polarization of macrophages. (A) RT-qPCR was used to detect the expression of M2 phenotype marker genes (CD206, CD163, IL-10) in co cultured macrophages; (B) Flow cytometry was used to detect the percentage of M2 macrophages in co cultured macrophages. N = 3, ns p > 0.05, **p < 0.01, ****p < 0.0001.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

Techniques: Quantitative RT-PCR, Expressing, Marker, Cell Culture, Flow Cytometry

Journal: Frontiers in Oncology

Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

doi: 10.3389/fonc.2025.1538649

Figure Lengend Snippet: OSMR activates JAK/STAT3/CCL-2 pathway to promote tumor growth and M2 polarization of macrophages. (A) WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis. (B) Images, tumor volume, and weight of subcutaneous tumors in each group of mice; (C) RT-qPCR was used to detect the expression of M2 phenotype marker genes (CD206, CD163, IL-10) in macrophages in tumor tissues; (D) Flow cytometry is used to detect the percentage of M2 macrophages in tumor tissue macrophages. N = 6, ns p > 0.05, ****p < 0.0001.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

Techniques: Expressing, Quantitative RT-PCR, Marker, Flow Cytometry